A new method for identification of Trichomonas vaginalis by fluorescent DNA in situ hybridization.

نویسندگان

  • R Muresu
  • S Rubino
  • P Rizzu
  • A Baldini
  • M Colombo
  • P Cappuccinelli
چکیده

The protozoan flagellate Trichomonas vaginalis is responsible for human trichomoniasis, one of the most widespread sexually transmitted diseases in the world. Several methods are currently used for laboratory diagnosis, including direct microscopic observation, cell culture, immunological techniques, and more recently, DNA probing and gene amplification. This report describes an in situ hybridization technique with specific DNA probes labeled with either biotin, rhodamine, or fluorescein for detection of T. vaginalis with fluorescence microscopy. Repetitive DNA sequences were evident in the nuclei of the protozoa as intensively fluorescent regions, giving a spotted pattern. No cross-reactivity between the probes and the DNAs of mammalian cells, yeasts, or bacteria was noted. This technique is potentially useful for the diagnosis of human trichomoniasis in clinical samples.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification and Evaluation of Trichomonas vaginalis in Wesley Guild Hospital Ilesha, Nigeria

The Identification and evaluation of Trichomonas vaginalis among women was performed over a period of three months in Ilesha. The aim of this study was to determine the prevalence of Trichomonas vaginalis in women in relation to different samples collected and level of education. 310 samples from high vaginal, endocervical and urethral regions of female patients were analyzed using Gram stainin...

متن کامل

Evaluation of Discontinues Puresperm Gradient in Separation of X And Y Bearing Human Spermatozoa by Fluorescent in Situ Hybridization (FISH)

Introduction: To evaluate the 8 layer discontinues puresperm gradient in separation of human spermatozoa according to sex chromosomes by fluorescent in situ hybridization (FISH). Material and Methods: This study was carried out on three patient referring to Royan fertility and infertility center. Semen analysis was assessed according to WHO criteria. Each sample was divided into two aliquots, ...

متن کامل

Prevalence of Trichomonas Vaginalis in Diabetic Females

Abstract Background and Objective: Trichomonas vaginalis is a pathogenic agent known worldwide and affects about 180 million annually. We aimed to investigate the prevalence of Trichomonas vaginalis in diabetic patients referred to diabetic research center. Material and Methods: In this descriptive-analytic study, 215 women referring to the Diabetic Center in Kermanshah Province were invest...

متن کامل

دورگه‌سازی در محل؛ اصول و کاربردها : مقاله مروری

In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...

متن کامل

Fluorescent in Situ Hybridization and Real-Time Quantitative Polymerase Chain Reaction to Evaluate HER-2/neu Status in Breast Cancer

Background:Breast cancer remains the most common and second lethal cancer in females. HER-2/neu is one of the most important amplified oncogene in breast cancer. The amplification of HER-2 is correlated with decreased survival, metastasis, and early recurrence.  The amplification of HER-2/neu gene and synthesis of th...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 32 4  شماره 

صفحات  -

تاریخ انتشار 1994